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Semaglutide Reconstitution Calculator

Semaglutide vials are small (2 to 10 mg) and entries are fractions of a milligram, so the risk is a draw that is too small to read. Presets below keep the numbers in the readable range.

Peptide5 mg
Water2 mL
Dose250 mcg
Syringe30U
Draw10U

Peptide in the vial

5 mg
mg

Bacteriostatic water added

2 mL
mL

More water = more units per dose (easier to measure). Less water = fewer units (fits smaller syringes).

Syringe

30U · 0.3 mL

Check the last printed number on your barrel: 30, 50 or 100. That is the capacity.

Dose you want

250 mcg

Vial price (optional)

–
$shows cost per dose

Supplies for your next vial

Everything around the vial, in one box, from the same people who built this calculator.

Reconstitution Supply Kit

  • 10 U-100 insulin syringes, 31G, 5/16" needle (the 30U size this calculator defaults to)
  • 50 sterile 70% isopropyl alcohol prep pads
  • 1-quart sharps container so used needles never go in the trash
  • Printed reconstitution and storage card

Does not include bacteriostatic water or peptides. Research and laboratory use. Must be 18+ to purchase. Not shipped to states that restrict non-prescription syringe sales.

$24.95 free US shipping · ships in 3 to 5 days
Reserve a kit (email, pay when it ships) Online checkout opens shortly. Reservations are first in line and there is no charge until the kit ships.

Common vial sizes and water amounts

VialWaterWhat that gives you
2 mg1 mL2 mg/mL; 0.25 mg = 12.5U
5 mg2 mL2.5 mg/mL; 0.25 mg = 10U
5 mg2.5 mL2 mg/mL; 0.5 mg = 25U
10 mg4 mL2.5 mg/mL; 1 mg = 40U

Worked examples

5 mg vial + 2 mL water, entering 250 mcg
Concentration 2.5 mg/mL → 25 mcg per unit → draw 10 units (0.1 mL) on a 30U syringe. That vial holds about 20 such draws.
2 mg vial + 1 mL water, entering 250 mcg
Concentration 2 mg/mL → 20 mcg per unit → draw 12.5 units (0.125 mL) on a 30U syringe. That vial holds about 8 such draws.
10 mg vial + 4 mL water, entering 1 mg
Concentration 2.5 mg/mL → 25 mcg per unit → draw 40 units (0.4 mL) on a 50U syringe. That vial holds about 10 such draws.

Notes for this calculator

Aim for a concentration around 2 to 2.5 mg/mL: a 0.25 mg entry lands at 10 to 12.5 units, which is easy to read on a 30U syringe, and a 1 mg entry still fits a 50U. Mixing a 5 mg vial with only 1 mL puts 0.25 mg at 5 units, where a half-unit error is 10% of the draw.

Add the vial price on the left and the card shows cost per draw. For semaglutide that number is usually the one people are actually deciding on.

Vials for this calculator: see what ThePeptideStore carries. Supplies around the vial are in the kit above.

Common questions

How many units is 0.25 mg of semaglutide?

At 2.5 mg/mL (5 mg in 2 mL) it is 10 units. At 2 mg/mL it is 12.5 units. At 5 mg/mL (5 mg in 1 mL) it is only 5 units, which is hard to measure.

How much bacteriostatic water for a 5 mg semaglutide vial?

2 mL gives 2.5 mg/mL and puts a 0.25 mg entry at 10 units. 2.5 mL gives 2 mg/mL. Both keep the draw readable.

How many draws are in a 5 mg vial?

5 mg at 0.25 mg per entry is 20 draws; at 0.5 mg it is 10. The calculator shows doses per vial and cost per draw.

Does the calculator recommend a dose?

No. You enter the dose your protocol calls for and it converts that into syringe units, mL, and doses per vial. It never suggests what to take.

Why is my draw too big for the syringe?

The concentration is low for that dose. Either use a larger U-100 syringe or mix the next vial with less water. The calculator shows the exact water amount that would fit your syringe.

About this tool. Built and maintained by the team at ThePeptideStore. The arithmetic is the standard U-100 reconstitution formula (concentration = mg ÷ mL; mcg per unit = concentration × 10; units = dose ÷ mcg per unit) and every result is recomputed live in your browser; nothing you enter is sent to a server. Reviewed 2026-09-02. This calculator does arithmetic on numbers you type. It is not medical advice and does not recommend any dose.
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